A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.
Identifieur interne : 000273 ( Ncbi/Merge ); précédent : 000272; suivant : 000274A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.
Auteurs : Lihua Zhou [République populaire de Chine] ; Huimin Deng ; Qinying Deng ; Shankai ZhaoSource :
- Rapid communications in mass spectrometry : RCM [ 0951-4198 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN (analyse), ADN (génétique), Acides picoliniques, Données de séquences moléculaires, Oligodésoxyribonucléotides (analyse), Oligodésoxyribonucléotides (génétique), Pyrazinamide (analogues et dérivés), Reproductibilité des résultats, Solutions, Spectrométrie de masse MALDI (instrumentation), Séquence nucléotidique.
- MESH :
- analogues et dérivés : Pyrazinamide.
- analyse : ADN, Oligodésoxyribonucléotides.
- génétique : ADN, Oligodésoxyribonucléotides.
- instrumentation : Acides picoliniques, Données de séquences moléculaires, Reproductibilité des résultats, Solutions, Spectrométrie de masse MALDI, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, DNA (analysis), DNA (genetics), Molecular Sequence Data, Oligodeoxyribonucleotides (analysis), Oligodeoxyribonucleotides (genetics), Picolinic Acids, Pyrazinamide (analogs & derivatives), Reproducibility of Results, Solutions, Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (instrumentation).
- MESH :
- chemical , analogs & derivatives : Pyrazinamide.
- chemical , analysis : DNA, Oligodeoxyribonucleotides.
- chemical , genetics : DNA, Oligodeoxyribonucleotides.
- instrumentation : Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization.
- Base Sequence, Molecular Sequence Data, Picolinic Acids, Reproducibility of Results, Solutions.
Abstract
A mixed matrix of 3-hydroxypicolinic acid (3-HPA) and pyrazinecarboxylic acid (PCA) was used for analysis of a variety of synthetic oligodeoxynucleotides ranging in length from 8-108-mers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The experimental results showed that DNA segments with masses in the range from 5000 to 10,500 Da can be analyzed with high resolution (isotopic peaks resolved) when 3-HPA/PCA was prepared by mixing saturated 3-HPA solution and saturated PCA solution (in 50% of a 0.5 mol/L solution of diammonium hydrogen citrate plus 50% acetonitrile) at the volume ratio of 4:1. Each component of a mixture of d(A)1-10 gave a well-resolved peak. Moreover, when 3-HPA/PCA was used to analyze two mixtures, one containing two 23-mer DNA segments with a 9 Da difference (A and T) and the other with a 7 Da difference (AA and TG), the two 23-mer ion peaks were well separated from each other and an isotopically resolved spectrum of each component was recorded. Under the identical experimental conditions, the sample-to-sample reproducibility, resolution, signal-to-noise ratio and the tolerance to metal salts, with 3-HPA/PCA, were superior to those observed using 3-HPA alone for analysis of DNA segments.
DOI: 10.1002/rcm.1404
PubMed: 15052561
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002396
- to stream PubMed, to step Curation: 002396
- to stream PubMed, to step Checkpoint: 002287
Links to Exploration step
pubmed:15052561Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.</title>
<author><name sortKey="Zhou, Lihua" sort="Zhou, Lihua" uniqKey="Zhou L" first="Lihua" last="Zhou">Lihua Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong, P.R. China.</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong</wicri:regionArea>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Deng, Huimin" sort="Deng, Huimin" uniqKey="Deng H" first="Huimin" last="Deng">Huimin Deng</name>
</author>
<author><name sortKey="Deng, Qinying" sort="Deng, Qinying" uniqKey="Deng Q" first="Qinying" last="Deng">Qinying Deng</name>
</author>
<author><name sortKey="Zhao, Shankai" sort="Zhao, Shankai" uniqKey="Zhao S" first="Shankai" last="Zhao">Shankai Zhao</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15052561</idno>
<idno type="pmid">15052561</idno>
<idno type="doi">10.1002/rcm.1404</idno>
<idno type="wicri:Area/PubMed/Corpus">002396</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002396</idno>
<idno type="wicri:Area/PubMed/Curation">002396</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002396</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002287</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002287</idno>
<idno type="wicri:Area/Ncbi/Merge">000273</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.</title>
<author><name sortKey="Zhou, Lihua" sort="Zhou, Lihua" uniqKey="Zhou L" first="Lihua" last="Zhou">Lihua Zhou</name>
<affiliation wicri:level="1"><nlm:affiliation>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong, P.R. China.</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong</wicri:regionArea>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Deng, Huimin" sort="Deng, Huimin" uniqKey="Deng H" first="Huimin" last="Deng">Huimin Deng</name>
</author>
<author><name sortKey="Deng, Qinying" sort="Deng, Qinying" uniqKey="Deng Q" first="Qinying" last="Deng">Qinying Deng</name>
</author>
<author><name sortKey="Zhao, Shankai" sort="Zhao, Shankai" uniqKey="Zhao S" first="Shankai" last="Zhao">Shankai Zhao</name>
</author>
</analytic>
<series><title level="j">Rapid communications in mass spectrometry : RCM</title>
<idno type="ISSN">0951-4198</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term>
<term>DNA (analysis)</term>
<term>DNA (genetics)</term>
<term>Molecular Sequence Data</term>
<term>Oligodeoxyribonucleotides (analysis)</term>
<term>Oligodeoxyribonucleotides (genetics)</term>
<term>Picolinic Acids</term>
<term>Pyrazinamide (analogs & derivatives)</term>
<term>Reproducibility of Results</term>
<term>Solutions</term>
<term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization (instrumentation)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (analyse)</term>
<term>ADN (génétique)</term>
<term>Acides picoliniques</term>
<term>Données de séquences moléculaires</term>
<term>Oligodésoxyribonucléotides (analyse)</term>
<term>Oligodésoxyribonucléotides (génétique)</term>
<term>Pyrazinamide (analogues et dérivés)</term>
<term>Reproductibilité des résultats</term>
<term>Solutions</term>
<term>Spectrométrie de masse MALDI (instrumentation)</term>
<term>Séquence nucléotidique</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analogs & derivatives" xml:lang="en"><term>Pyrazinamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA</term>
<term>Oligodeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA</term>
<term>Oligodeoxyribonucleotides</term>
</keywords>
<keywords scheme="MESH" qualifier="analogues et dérivés" xml:lang="fr"><term>Pyrazinamide</term>
</keywords>
<keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN</term>
<term>Oligodésoxyribonucléotides</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN</term>
<term>Oligodésoxyribonucléotides</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term>
<term>Molecular Sequence Data</term>
<term>Picolinic Acids</term>
<term>Reproducibility of Results</term>
<term>Solutions</term>
</keywords>
<keywords scheme="MESH" qualifier="instrumentation" xml:lang="fr"><term>Acides picoliniques</term>
<term>Données de séquences moléculaires</term>
<term>Reproductibilité des résultats</term>
<term>Solutions</term>
<term>Spectrométrie de masse MALDI</term>
<term>Séquence nucléotidique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">A mixed matrix of 3-hydroxypicolinic acid (3-HPA) and pyrazinecarboxylic acid (PCA) was used for analysis of a variety of synthetic oligodeoxynucleotides ranging in length from 8-108-mers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The experimental results showed that DNA segments with masses in the range from 5000 to 10,500 Da can be analyzed with high resolution (isotopic peaks resolved) when 3-HPA/PCA was prepared by mixing saturated 3-HPA solution and saturated PCA solution (in 50% of a 0.5 mol/L solution of diammonium hydrogen citrate plus 50% acetonitrile) at the volume ratio of 4:1. Each component of a mixture of d(A)1-10 gave a well-resolved peak. Moreover, when 3-HPA/PCA was used to analyze two mixtures, one containing two 23-mer DNA segments with a 9 Da difference (A and T) and the other with a 7 Da difference (AA and TG), the two 23-mer ion peaks were well separated from each other and an isotopically resolved spectrum of each component was recorded. Under the identical experimental conditions, the sample-to-sample reproducibility, resolution, signal-to-noise ratio and the tolerance to metal salts, with 3-HPA/PCA, were superior to those observed using 3-HPA alone for analysis of DNA segments.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15052561</PMID>
<DateCompleted><Year>2004</Year>
<Month>07</Month>
<Day>21</Day>
</DateCompleted>
<DateRevised><Year>2013</Year>
<Month>11</Month>
<Day>21</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0951-4198</ISSN>
<JournalIssue CitedMedium="Print"><Volume>18</Volume>
<Issue>7</Issue>
<PubDate><Year>2004</Year>
</PubDate>
</JournalIssue>
<Title>Rapid communications in mass spectrometry : RCM</Title>
<ISOAbbreviation>Rapid Commun. Mass Spectrom.</ISOAbbreviation>
</Journal>
<ArticleTitle>A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides.</ArticleTitle>
<Pagination><MedlinePgn>787-94</MedlinePgn>
</Pagination>
<Abstract><AbstractText>A mixed matrix of 3-hydroxypicolinic acid (3-HPA) and pyrazinecarboxylic acid (PCA) was used for analysis of a variety of synthetic oligodeoxynucleotides ranging in length from 8-108-mers by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry. The experimental results showed that DNA segments with masses in the range from 5000 to 10,500 Da can be analyzed with high resolution (isotopic peaks resolved) when 3-HPA/PCA was prepared by mixing saturated 3-HPA solution and saturated PCA solution (in 50% of a 0.5 mol/L solution of diammonium hydrogen citrate plus 50% acetonitrile) at the volume ratio of 4:1. Each component of a mixture of d(A)1-10 gave a well-resolved peak. Moreover, when 3-HPA/PCA was used to analyze two mixtures, one containing two 23-mer DNA segments with a 9 Da difference (A and T) and the other with a 7 Da difference (AA and TG), the two 23-mer ion peaks were well separated from each other and an isotopically resolved spectrum of each component was recorded. Under the identical experimental conditions, the sample-to-sample reproducibility, resolution, signal-to-noise ratio and the tolerance to metal salts, with 3-HPA/PCA, were superior to those observed using 3-HPA alone for analysis of DNA segments.</AbstractText>
<CopyrightInformation>Copyright 2004 John Wiley & Sons, Ltd.</CopyrightInformation>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Lihua</ForeName>
<Initials>L</Initials>
<AffiliationInfo><Affiliation>Instrumental Analysis & Research Center, Sun Yat-Sen University, Guangzhou 510275, Guangdong, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Deng</LastName>
<ForeName>Huimin</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Deng</LastName>
<ForeName>Qinying</ForeName>
<Initials>Q</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhao</LastName>
<ForeName>Shankai</ForeName>
<Initials>S</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>England</Country>
<MedlineTA>Rapid Commun Mass Spectrom</MedlineTA>
<NlmUniqueID>8802365</NlmUniqueID>
<ISSNLinking>0951-4198</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D009838">Oligodeoxyribonucleotides</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D010848">Picolinic Acids</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D012996">Solutions</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>2KNI5N06TI</RegistryNumber>
<NameOfSubstance UI="D011718">Pyrazinamide</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>2WB23298SP</RegistryNumber>
<NameOfSubstance UI="C005296">pyrazinoic acid</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>874-24-8</RegistryNumber>
<NameOfSubstance UI="C049246">3-hydroxypicolinic acid</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>9007-49-2</RegistryNumber>
<NameOfSubstance UI="D004247">DNA</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004247" MajorTopicYN="N">DNA</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="N">analysis</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009838" MajorTopicYN="N">Oligodeoxyribonucleotides</DescriptorName>
<QualifierName UI="Q000032" MajorTopicYN="Y">analysis</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010848" MajorTopicYN="Y">Picolinic Acids</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011718" MajorTopicYN="Y">Pyrazinamide</DescriptorName>
<QualifierName UI="Q000031" MajorTopicYN="Y">analogs & derivatives</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015203" MajorTopicYN="N">Reproducibility of Results</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D012996" MajorTopicYN="N">Solutions</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019032" MajorTopicYN="N">Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization</DescriptorName>
<QualifierName UI="Q000295" MajorTopicYN="Y">instrumentation</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year>
<Month>3</Month>
<Day>31</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2004</Year>
<Month>7</Month>
<Day>22</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2004</Year>
<Month>3</Month>
<Day>31</Day>
<Hour>5</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15052561</ArticleId>
<ArticleId IdType="doi">10.1002/rcm.1404</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
</list>
<tree><noCountry><name sortKey="Deng, Huimin" sort="Deng, Huimin" uniqKey="Deng H" first="Huimin" last="Deng">Huimin Deng</name>
<name sortKey="Deng, Qinying" sort="Deng, Qinying" uniqKey="Deng Q" first="Qinying" last="Deng">Qinying Deng</name>
<name sortKey="Zhao, Shankai" sort="Zhao, Shankai" uniqKey="Zhao S" first="Shankai" last="Zhao">Shankai Zhao</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Zhou, Lihua" sort="Zhou, Lihua" uniqKey="Zhou L" first="Lihua" last="Zhou">Lihua Zhou</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/MersV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000273 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000273 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= MersV1 |flux= Ncbi |étape= Merge |type= RBID |clé= pubmed:15052561 |texte= A mixed matrix of 3-hydroxypicolinic acid and pyrazinecarboxylic acid for matrix-assisted laser desorption/ionization time-of-flight mass spectrometry of oligodeoxynucleotides. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:15052561" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a MersV1
This area was generated with Dilib version V0.6.33. |